The public health and international development communities know that gender strongly influences HIV vulnerability and how people respond to the epidemic. There is growing recognition that using multiple approaches in HIV/AIDS programming is more effective than single strategies. Recognizing the many links between gender and the continuing global AIDS epidemic, the U.S. President’s Emergency Plan for AIDS Relief (PEPFAR) advocates for the inclusion of gender strategies in HIV prevention, treatment, and care and support programs around the world. Despite increased understanding of the link between gender and HIV and, more recently, the value of using multiple gender strategies to mitigate women’s and men’s vulnerability, little is known about how HIV programs are applying these insights to improve programs and services. To expand this knowledge base, PEPFAR’s Gender Technical Working Group commissioned AIDSTAR-One to compile a compendium of HIV prevention, treatment, and care and support programs in sub-Saharan Africa that are integrating multiple gender strategies into their work. The resulting compendium describes how 31 programs in Africa are using gender strategies to improve HIV services and reduce vulnerability to HIV infection. The compendium provides examples of how strategies are combined, where gaps exist, lessons learned, and common experiences across programs.